Police said the bus was carrying children and adults who are members of Colonial Hill Baptist Church. The bus was returning from summer camp in Michigan before it overturned about 4:15 p.m.
Witnesses said the bus was coming off of I-465 before trying to turn onto Keystone Ave., but didn't slow down.
Indianapolis Fire Department spokeswoman Lt. Rita Burris confirmed the fatalities and said survivors were being taken to area hospitals. Those injured include children and adults.